Hotspot

trnE-UUC-trnT-GGU

LSC · 30605-31175 · 571 bp

Back to Lagopsis

Pi 0.0035

MarkerSeek score 47.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 3
pi: 0.0035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	708-710 bp	1.0	80.2
trnE-UUC-trnT-GGU_p2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	687-689 bp	1.0	79.7
trnE-UUC-trnT-GGU_p3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	58.0	58.1	684-686 bp	1.0	79.6
trnE-UUC-trnT-GGU_p4	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	705-707 bp	1.0	79.0
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	673-675 bp	1.0	78.3