Hotspot

rpoB-trnC-GCA

LSC · 26703-27797 · 1095 bp

Back to Lagopsis

Pi 0.00122

MarkerSeek score 60.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 2
pi: 0.00122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCTGTCAAACCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1249-1260 bp	1.0	78.2
rpoB-trnC-GCA_p2	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1250-1261 bp	1.0	78.1
rpoB-trnC-GCA_p3	GTTCTTCTGTCAAACCCCGA	TTTGGTAATCAGGCGACACC	50.0	50.0	57.8	58.2	1268-1279 bp	1.0	77.3
rpoB-trnC-GCA_p4	AGTTCTTCTGTCAAACCCCG	TTTGGTAATCAGGCGACACC	50.0	50.0	57.7	58.2	1269-1280 bp	1.0	77.3
rpoB-trnC-GCA_p5	GTTCTTCTGTCAAACCCCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1231-1242 bp	1.0	76.0