Hotspot

atpF-atpH

LSC · 12832-13201 · 370 bp

Back to Lagopsis

Pi 0.00725

MarkerSeek score 59.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 4
pi: 0.00725
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	CGGAGGGAAAAATACGAGGT	45.0	50.0	57.9	57.3	518-521 bp	1.0	75.1
atpF-atpH_p2	TGGCCCAAAGAAACGAAAGA	CGAGGCGGAGGGAAAAATAC	45.0	55.0	57.9	58.7	523-526 bp	1.0	75.0
atpF-atpH_p3	TGGCCCAAAGAAACGAAAGA	GAGGCGGAGGGAAAAATACG	45.0	55.0	57.9	58.7	522-525 bp	1.0	75.0
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	GCGGAGGGAAAAATACGAGG	45.0	55.0	57.9	58.7	519-522 bp	1.0	75.0
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	GGCGGAGGGAAAAATACGAG	45.0	55.0	57.9	58.7	520-523 bp	1.0	75.0