Hotspot

trnS-GCU-trnS-CGA

LSC · 8218-8899 · 682 bp

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Pi 0.00196

MarkerSeek score 61.20

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 2
pi: 0.00196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	730-737 bp	1.0	62.1
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	729-736 bp	1.0	62.1
trnS-GCU-trnS-CGA_p3	ACGGAAAGAGAGGGATTCGA	ACCACTAAACTATACCCGCT	50.0	45.0	58.2	55.6	784-791 bp	1.0	60.9
trnS-GCU-trnS-CGA_p4	AGCAATCCGACGCTTTAGTC	ACCACTAAACTATACCCGCT	50.0	45.0	58.4	55.6	732-739 bp	1.0	59.3
trnS-GCU-trnS-CGA_p5	CGACGCTTTAGTCCACTCAG	ACCACTAAACTATACCCGCT	55.0	45.0	58.4	55.6	725-732 bp	1.0	59.1