Hotspot

ycf1

SSC · 121542-127058 · 5517 bp

Back to Lagochilus

Pi 0.0062

MarkerSeek score 50.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5517
variable_sites: 129
pi: 0.0062
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTCGAAATAGACTGAATTGGAGT	GAAAAACAACAAATGGTAAAAATG	37.5	25.0	57.7	52.9	255 bp	1.0	42.8
ycf1_p2	ACTCGAAATAGACTGAATTGGAG	GAAAAACAACAAATGGTAAAAATG	39.1	25.0	56.1	52.9	255 bp	1.0	42.8
ycf1_p3	CTCGAAATAGACTGAATTGGAGT	GAAAAACAACAAATGGTAAAAATG	39.1	25.0	56.1	52.9	254 bp	1.0	42.8
ycf1_p4	ACTCGAAATAGACTGAATTGGA	GAAAAACAACAAATGGTAAAAATG	36.4	25.0	54.9	52.9	255 bp	1.0	42.8
ycf1_p5	TCGAAATAGACTGAATTGGAGT	GAAAAACAACAAATGGTAAAAATG	36.4	25.0	54.9	52.9	253 bp	1.0	42.8