Hotspot

rpoB

LSC · 23654-26872 · 3219 bp

Back to Lagochilus

Pi 0.00163

MarkerSeek score 57.00

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3219
variable_sites: 21
pi: 0.00163
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GTATTCATCTCTGCCGGGTG	CTAACAGGGCGAACTGCTAG	55.0	55.0	58.1	58.1	228 bp	1.0	79.4
rpoB_p2	AGTATTCATCTCTGCCGGGT	CTAACAGGGCGAACTGCTAG	50.0	55.0	58.2	58.1	229 bp	1.0	78.8
rpoB_p3	TATTCATCTCTGCCGGGTGA	CTAACAGGGCGAACTGCTAG	50.0	55.0	58.2	58.1	227 bp	1.0	78.8
rpoB_p4	TCATCTCTGCCGGGTGATAA	CTAACAGGGCGAACTGCTAG	50.0	55.0	58.2	58.1	224 bp	1.0	78.8
rpoB_p5	TTCATCTCTGCCGGGTGATA	CTAACAGGGCGAACTGCTAG	50.0	55.0	58.2	58.1	225 bp	1.0	78.8