Hotspot

petD

LSC · 75174-76407 · 1234 bp

Back to Lagochilus

Pi 0.00676

MarkerSeek score 55.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1234
variable_sites: 34
pi: 0.00676
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAATTCTCCGA	TCCCTAGATACATACGGCGT	45.0	50.0	56.2	57.4	1310 bp	0.8	54.3
petD_p2	TGAAGGGAATTCTCCGAAGAG	TCCCTAGATACATACGGCGT	47.6	50.0	57.1	57.4	1307 bp	0.8	53.9
petD_p3	AGTTGAAGGGAATTCTCCGA	CCCTAGATACATACGGCGTG	45.0	55.0	56.2	57.4	1309 bp	0.8	53.9
petD_p4	TGAAGGGAATTCTCCGAAGAG	CCCTAGATACATACGGCGTG	47.6	55.0	57.1	57.4	1306 bp	0.8	53.4
petD_p5	AGTTGAAGGGAATTCTCCGAAG	TCCCTAGATACATACGGCGT	45.5	50.0	58.1	57.4	1310 bp	0.8	52.6