Hotspot

ccsA

SSC · 112115-113086 · 972 bp

Back to Lagochilus

Pi 0.00668

MarkerSeek score 42.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 20
pi: 0.00668
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ATCTCGCCATCAATCCACAC	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	2100-2119 bp	1.0	82.3
ccsA_p2	GTGAAATCGGTAGACACGCT	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1679-1693 bp	1.0	82.1
ccsA_p3	TGAAATCGGTAGACACGCTG	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1678-1692 bp	1.0	82.1
ccsA_p4	GTGAAATCGGTAGACACGCT	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1678-1692 bp	1.0	82.0
ccsA_p5	TGAAATCGGTAGACACGCTG	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1677-1691 bp	1.0	82.0