Hotspot

rps15-ycf1

SSC · 121117-121541 · 425 bp

Back to Lagochilus

Pi 0.00915

MarkerSeek score 51.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 425
variable_sites: 13
pi: 0.00915
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	CGATGGGATGAGGCTTTGAA	50.0	50.0	58.0	58.0	1310-1319 bp	1.0	82.5
rps15-ycf1_p2	CCAATACCGGTTCGCAGTTA	CGATGGGATGAGGCTTTGAA	50.0	50.0	58.0	58.0	1625-1633 bp	1.0	82.0
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	TCAATTGCGATGGGATGAGG	50.0	50.0	58.0	58.0	1317-1326 bp	0.8	73.6
rps15-ycf1_p4	AGTCGTTGACGTTTTCCCAA	TCAATTGCGATGGGATGAGG	45.0	50.0	58.0	58.0	1313-1322 bp	0.8	73.4
rps15-ycf1_p5	CCAATACCGGTTCGCAGTTA	TCAATTGCGATGGGATGAGG	50.0	50.0	58.0	58.0	1632-1640 bp	0.8	73.3