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psbE-petL

LSC · 63913-64854 · 942 bp

Back to Lagochilus

Pi 0.00889

MarkerSeek score 64.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 33
pi: 0.00889
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1128-1157 bp	1.0	76.0
psbE-petL_p2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1141-1170 bp	1.0	75.7
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1051-1080 bp	1.0	75.4
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1137-1166 bp	1.0	75.0
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1136-1165 bp	1.0	74.9