Hotspot

petA-psbJ

LSC · 62083-63126 · 1044 bp

Back to Lagochilus

Pi 0.012

MarkerSeek score 74.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1044
variable_sites: 41
pi: 0.012
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGGGCTTTTGGTCTTCTTGG	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	942-1187 bp	1.0	83.0
petA-psbJ_p2	CCAAGGGCTTTTGGTCTTCT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	945-1190 bp	1.0	83.0
petA-psbJ_p3	TCCAAGGGCTTTTGGTCTTC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	946-1191 bp	1.0	83.0
petA-psbJ_p4	AGGGCTTTTGGTCTTCTTGG	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.0	57.4	941-1186 bp	1.0	82.5
petA-psbJ_p5	CCAAGGGCTTTTGGTCTTCT	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.0	57.4	944-1189 bp	1.0	82.5