Hotspot

ycf4-cemA

LSC · 59654-60213 · 560 bp

Back to Lagochilus

Pi 0.00401

MarkerSeek score 58.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 560
variable_sites: 8
pi: 0.00401
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGACCCGTACTGATGAG	GAAATCCACCAGGGCAGAAA	55.0	50.0	58.1	58.1	728-729 bp	1.0	79.8
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.1	58.1	658-659 bp	1.0	79.5
ycf4-cemA_p3	GAACAAAAAGCCGCGGAATT	GAAATCCACCAGGGCAGAAA	45.0	50.0	58.2	58.1	687-688 bp	1.0	78.7
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.7	58.1	661-662 bp	1.0	77.9
ycf4-cemA_p5	CGTTGACCCGTACTGATGAG	TCCACCAGGGCAGAAATACT	55.0	50.0	58.1	58.3	724-725 bp	1.0	77.7