Hotspot

atpB-rbcL

LSC · 53776-54567 · 792 bp

Back to Lagochilus

Pi 0.0062

MarkerSeek score 63.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 15
pi: 0.0062
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGGCCCGATTATTTGGATGA	AACACCCGCTTTGAATCCAA	50.0	45.0	58.1	58.3	941-961 bp	1.0	79.7
atpB-rbcL_p2	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	964-984 bp	1.0	79.2
atpB-rbcL_p3	AGTACCGGCCCGATTATTTG	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	946-966 bp	1.0	78.5
atpB-rbcL_p4	ATTTGGATGATACGCCCCTG	AACACCCGCTTTGAATCCAA	50.0	45.0	57.7	58.3	931-951 bp	1.0	78.4
atpB-rbcL_p5	GATGATACGCCCCTGGTTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	926-946 bp	0.9	75.9