Hotspot

trnG-GCC-trnfM-CAU

LSC · 36274-36417 · 144 bp

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Pi 0.0148
MarkerSeek score 57.50
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
144
variable_sites
7
pi
0.0148
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 208-214 bp 1.0 82.6
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 209-215 bp 1.0 82.6
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 244-250 bp 1.0 82.2
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 245-251 bp 1.0 82.2
trnG-GCC-trnfM-CAU_p5 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 285-291 bp 1.0 81.5