Hotspot

trnT-CGU-psbD

LSC · 31447-32700 · 1254 bp

Back to Lagochilus

Pi 0.0063

MarkerSeek score 55.10

Primer available yes

Label trnT-CGU-psbD

Feature Metadata

label_name: trnT-CGU-psbD
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 24
pi: 0.0063
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-CGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	986-1415 bp	1.0	84.1
trnT-CGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	989-1418 bp	1.0	83.9
trnT-CGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	976-1405 bp	1.0	83.0
trnT-CGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	979-1408 bp	1.0	82.8
trnT-CGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	963-1392 bp	1.0	82.4