Hotspot

rpoB-trnC-GCA

LSC · 26873-27959 · 1087 bp

Back to Lagochilus

Pi 0.00452

MarkerSeek score 67.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 19
pi: 0.00452
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGACATTGCCTCATTCGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	58.0	58.1	1173-1208 bp	1.0	82.8
rpoB-trnC-GCA_p2	AGACATTGCCTCATTCGCAT	TTTGGTAATCAGGCGACACC	45.0	50.0	58.0	58.2	1192-1227 bp	1.0	81.9
rpoB-trnC-GCA_p3	GTTCTTCTGTCAAACCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1246-1281 bp	1.0	81.0
rpoB-trnC-GCA_p4	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1247-1282 bp	1.0	81.0
rpoB-trnC-GCA_p5	AGACATTGCCTCATTCGCAT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	58.0	58.4	1155-1190 bp	1.0	80.7