Hotspot

atpH-atpI

LSC · 13598-14558 · 961 bp

Back to Lagochilus

Pi 0.00816

MarkerSeek score 63.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 30
pi: 0.00816
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1086-1110 bp	1.0	83.0
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	1102-1126 bp	1.0	82.1
atpH-atpI_p3	AACGGAAGCGGCAGAAATTA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1084-1108 bp	1.0	81.9
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1087-1111 bp	1.0	81.9
atpH-atpI_p5	TAACGGAAGCGGCAGAAATT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1085-1109 bp	1.0	81.9