Hotspot

rps16-trnQ-UUG

LSC · 6085-7097 · 1013 bp

Back to Lagochilus

Pi 0.0084
MarkerSeek score 66.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1013
variable_sites
31
pi
0.0084
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAAATTGAAATGGGGCGTGG 50.0 50.0 57.9 58.0 933-1174 bp 1.0 85.5
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 875-1116 bp 1.0 85.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 916-1157 bp 1.0 85.0
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 858-1099 bp 1.0 84.6
rps16-trnQ-UUG_p5 CAACGGATCGTGTCCTTCAA GAAATTGAAATGGGGCGTGG 50.0 50.0 58.2 58.0 934-1175 bp 1.0 84.5