Hotspot

trnK-UUU-rps16

LSC · 4213-4940 · 728 bp

Back to Lagochilus

Pi 0.00733

MarkerSeek score 69.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 19
pi: 0.00733
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGTAAAAGATTCGAGCCGCA	TTTCCTTGAAAGGGGTGCTC	45.0	50.0	57.9	58.0	873-961 bp	1.0	83.3
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	849-937 bp	1.0	82.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	848-936 bp	1.0	82.7
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.3	58.0	858-946 bp	1.0	81.8
trnK-UUU-rps16_p5	AGATTCGAGCCGCACTTAAA	TTTCCTTGAAAGGGGTGCTC	45.0	50.0	57.6	58.0	867-955 bp	1.0	81.0