Hotspot

trnH-GUG-psbA

LSC · 76-333 · 258 bp

Back to Lagochilus

Pi 0.0223

MarkerSeek score 68.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 22
pi: 0.0223
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	349-387 bp	1.0	86.9
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	351-389 bp	1.0	86.8
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	363-401 bp	1.0	86.7
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	362-400 bp	1.0	86.7
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	364-402 bp	1.0	86.6