Hotspot

rpl32-trnL-UAG

SSC · 112824-113491 · 668 bp

Back to Lagerstroemia

Pi 0.0262

MarkerSeek score 71.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 668
variable_sites: 48
pi: 0.0262
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	758-800 bp	1.0	87.7
rpl32-trnL-UAG_p2	GCGTTGAAGGCTTTTTCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	759-801 bp	1.0	87.7
rpl32-trnL-UAG_p3	GCGTTGAAGGCTTTTTCGTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	768-810 bp	1.0	86.7
rpl32-trnL-UAG_p4	GCGTTGAAGGCTTTTTCGTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.2	57.8	767-809 bp	1.0	86.1
rpl32-trnL-UAG_p5	GCGTTGAAGGCTTTTTCGTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.2	57.8	766-808 bp	1.0	86.1