Hotspot

ndhF-rpl32

SSC · 111895-112649 · 755 bp

Back to Lagerstroemia

Pi 0.0226

MarkerSeek score 63.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 755
variable_sites: 47
pi: 0.0226
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	CAAGTGAACCGATGGGGAAA	50.0	50.0	58.1	58.1	1075-1171 bp	1.0	86.9
ndhF-rpl32_p2	GAAGAGGGAATTGTGCCGAT	CAAGTGAACCGATGGGGAAA	50.0	50.0	58.0	58.1	1692-1788 bp	1.0	86.0
ndhF-rpl32_p3	GAAGAGGGAATTGTGCCGAT	TGTGACAAGTGAACCGATGG	50.0	50.0	58.0	58.1	1697-1793 bp	1.0	85.8
ndhF-rpl32_p4	TCGCGTGAACCAAAAACCTA	CAAGTGAACCGATGGGGAAA	45.0	50.0	58.0	58.1	1522-1553 bp	0.789474	73.5
ndhF-rpl32_p5	TAGGTCGCGTGAACCAAAAA	CAAGTGAACCGATGGGGAAA	45.0	50.0	58.0	58.1	1526-1557 bp	0.789474	73.4