Hotspot

rrn16-trnI-GAU

IRb · 100893-101138 · 246 bp

Back to Lagerstroemia

Pi 0.0445

MarkerSeek score 63.00

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: IRb
length_bp: 246
variable_sites: 23
pi: 0.0445
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	GCTTGTGACTGGAGTGAAGT	GGCACAACGACGCAATTATC	50.0	50.0	57.8	58.2	363-401 bp	1.0	83.1
rrn16-trnI-GAU_p2	GCTTGTGACTGGAGTGAAGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	57.8	57.8	395-433 bp	1.0	82.7
rrn16-trnI-GAU_p3	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	370-408 bp	1.0	82.7
rrn16-trnI-GAU_p4	GCTTGTGACTGGAGTGAAGT	CGCTGATGAGCACATTGAAC	50.0	50.0	57.8	57.8	414-452 bp	1.0	82.4
rrn16-trnI-GAU_p5	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.7	57.8	389-427 bp	1.0	82.4