Hotspot

rbcL-accD

LSC · 56333-57088 · 756 bp

Back to Lagerstroemia

Pi 0.0138

MarkerSeek score 57.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 32
pi: 0.0138
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	GGCCACGCCTGTATTCTAAT	55.0	50.0	58.0	57.7	907-910 bp	1.0	80.5
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	GGCCACGCCTGTATTCTAAT	55.0	50.0	57.7	57.7	893-896 bp	1.0	78.6
rbcL-accD_p3	GGCTAGCAAATGGAGTCCTG	GGCCACGCCTGTATTCTAAT	55.0	50.0	58.3	57.7	905-908 bp	1.0	78.3
rbcL-accD_p4	AGGCTAGCAAATGGAGTCCT	GGCCACGCCTGTATTCTAAT	50.0	50.0	58.4	57.7	906-909 bp	1.0	77.6
rbcL-accD_p5	CGAGGCTAGCAAATGGAGTC	GGCCACGCCTGTATTCTAAT	55.0	50.0	58.4	57.7	908-911 bp	1.0	77.5