Pi
0.00971
MarkerSeek score
55.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 679-686 bp | 1.0 | 81.4 |
| trnT-UGU-trnL-UAA_p2 | AAATGCGGTGCTCTAACCTC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.3 | 58.0 | 651-658 bp | 1.0 | 81.4 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 687-694 bp | 1.0 | 80.9 |
| trnT-UGU-trnL-UAA_p4 | AAATGCGGTGCTCTAACCTC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.3 | 58.1 | 659-666 bp | 1.0 | 80.9 |
| trnT-UGU-trnL-UAA_p5 | GCATTACAAATGCGGTGCTC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.5 | 58.0 | 658-665 bp | 1.0 | 79.9 |