Hotspot

trnD-GUC-trnY-GUA

LSC · 29668-30120 · 453 bp

Back to Lagerstroemia

Pi 0.0187

MarkerSeek score 53.40

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 20
pi: 0.0187
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTTGATTCCGTCGGGACTG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	564-579 bp	1.0	82.8
trnD-GUC-trnY-GUA_p2	ATTTGATTCCGTCGGGACTG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	563-578 bp	1.0	82.8
trnD-GUC-trnY-GUA_p3	ATTTGATTCCGTCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	585-600 bp	1.0	81.7
trnD-GUC-trnY-GUA_p4	ATTTGATTCCGTCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	584-599 bp	1.0	81.5
trnD-GUC-trnY-GUA_p5	ATTTGATTCCGTCGGGACTG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.0	58.4	618-633 bp	1.0	81.0