Hotspot

trnK-UUU-rps16

LSC · 4073-4770 · 698 bp

Back to Lagerstroemia

Pi 0.0151

MarkerSeek score 53.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 34
pi: 0.0151
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	GCAATTGATGTGCGATCTCG	55.0	50.0	58.0	58.0	982-1042 bp	1.0	86.9
trnK-UUU-rps16_p2	ATTCATTCGACCCAACTCGG	GCAATTGATGTGCGATCTCG	50.0	50.0	58.0	58.0	1119-1179 bp	1.0	86.5
trnK-UUU-rps16_p3	CATTCGACCCAACTCGGAAT	GCAATTGATGTGCGATCTCG	50.0	50.0	58.0	58.0	1116-1176 bp	1.0	86.5
trnK-UUU-rps16_p4	TCAGGATCAGTCGTGGTCTT	GCAATTGATGTGCGATCTCG	50.0	50.0	58.1	58.0	978-1038 bp	1.0	86.4
trnK-UUU-rps16_p5	TTCAGGATCAGTCGTGGTCT	GCAATTGATGTGCGATCTCG	50.0	50.0	58.1	58.0	979-1039 bp	1.0	86.4