primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	73	202	197-205	1.000000	85.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	73	202	247-255	1.000000	84.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	73	202	260-268	1.000000	83.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	73	202	261-269	1.000000	83.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	73	202	262-270	1.000000	83.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	GCAATTGATGTGCGATCTCG	20	20	55.0	50.0	58.0	58.0	0.0	10.3	0.030377	4073	4770	982-1042	1.000000	86.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	ATTCATTCGACCCAACTCGG	GCAATTGATGTGCGATCTCG	20	20	50.0	50.0	58.0	58.0	0.0	10.3	0.038817	4073	4770	1119-1179	1.000000	86.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CATTCGACCCAACTCGGAAT	GCAATTGATGTGCGATCTCG	20	20	50.0	50.0	58.0	58.0	0.0	10.3	0.038817	4073	4770	1116-1176	1.000000	86.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCAGGATCAGTCGTGGTCTT	GCAATTGATGTGCGATCTCG	20	20	50.0	50.0	58.1	58.0	0.0	10.3	0.089910	4073	4770	978-1038	1.000000	86.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TTCAGGATCAGTCGTGGTCT	GCAATTGATGTGCGATCTCG	20	20	50.0	50.0	58.1	58.0	0.0	10.3	0.089910	4073	4770	979-1039	1.000000	86.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TATGTCTGTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.098913	5875	6390	557-677	1.000000	86.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.272396	5875	6390	542-662	1.000000	85.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.6	58.1	8.1	16.9	0.454608	5875	6390	546-666	1.000000	83.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CAAGTCGCACGTTGCTTTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.5	58.1	8.1	16.9	0.531415	5875	6390	548-668	1.000000	83.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TATGTCTGTCAAGTCGCACG	GGGTTTTGGTCCCGGTATTC	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.555955	5875	6390	578-698	1.000000	82.6
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	7638	8314	738-753	1.000000	57.1
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	7638	8314	737-752	1.000000	57.1
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	GAAAGGCTCGTACAACGGAT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	7638	8314	761-776	1.000000	57.0
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	GCTCGTACAACGGATTAGCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.1	55.5	0.0	0.0	3.618570	7638	8314	756-771	1.000000	56.4
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	7638	8314	792-807	1.000000	55.6
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	ATTTGATTCCGTCGGGACTG	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.0	57.8	12.3	0.0	0.263118	29668	30120	564-579	1.000000	82.8
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	ATTTGATTCCGTCGGGACTG	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.0	57.8	12.3	0.0	0.264669	29668	30120	563-578	1.000000	82.8
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	ATTTGATTCCGTCGGGACTG	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.0	58.4	12.3	0.0	0.375672	29668	30120	585-600	1.000000	81.7
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	ATTTGATTCCGTCGGGACTG	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.0	57.6	12.3	0.0	0.399349	29668	30120	584-599	1.000000	81.5
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	ATTTGATTCCGTCGGGACTG	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.0	58.4	12.3	0.0	0.436628	29668	30120	618-633	1.000000	81.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	45759	46359	679-686	1.000000	81.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAATGCGGTGCTCTAACCTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.265557	45759	46359	651-658	1.000000	81.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	45759	46359	687-694	1.000000	80.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAATGCGGTGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.330366	45759	46359	659-666	1.000000	80.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCATTACAAATGCGGTGCTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.5	58.0	8.7	0.0	0.455637	45759	46359	658-665	1.000000	79.9
rbcL-accD_p1	rbcL-accD	1	GAGGCTAGCAAATGGAGTCC	GGCCACGCCTGTATTCTAAT	20	20	55.0	50.0	58.0	57.7	18.2	0.0	0.295701	56333	57088	907-910	1.000000	80.5
rbcL-accD_p2	rbcL-accD	2	GAGTCCTGAACTAGCTGCTG	GGCCACGCCTGTATTCTAAT	20	20	55.0	50.0	57.7	57.7	13.4	0.0	0.548220	56333	57088	893-896	1.000000	78.6
rbcL-accD_p3	rbcL-accD	3	GGCTAGCAAATGGAGTCCTG	GGCCACGCCTGTATTCTAAT	20	20	55.0	50.0	58.3	57.7	18.2	0.0	0.582949	56333	57088	905-908	1.000000	78.3
rbcL-accD_p4	rbcL-accD	4	AGGCTAGCAAATGGAGTCCT	GGCCACGCCTGTATTCTAAT	20	20	50.0	50.0	58.4	57.7	18.2	0.0	0.666703	56333	57088	906-909	1.000000	77.6
rbcL-accD_p5	rbcL-accD	5	CGAGGCTAGCAAATGGAGTC	GGCCACGCCTGTATTCTAAT	20	20	55.0	50.0	58.4	57.7	18.2	0.0	0.679762	56333	57088	908-911	1.000000	77.5
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	TTCATACTCCGGGTTAGGCT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.208399	62827	63443	761-787	1.000000	83.5
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	GTTCATACTCCGGGTTAGGC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.274232	62827	63443	762-788	1.000000	83.0
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	TTCATACTCCGGGTTAGGCT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.324866	62827	63443	744-770	1.000000	82.7
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	TACTCCGGGTTAGGCTCATC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.337432	62827	63443	757-783	1.000000	82.5
petA-psbJ_p5	petA-psbJ	5	ATCCATTACGTGTCCAAGGC	TCATACTCCGGGTTAGGCTC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.337432	62827	63443	760-786	1.000000	82.5
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	GCTTGTGACTGGAGTGAAGT	GGCACAACGACGCAATTATC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.436522	100893	101138	363-401	1.000000	83.1
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	GCTTGTGACTGGAGTGAAGT	CTATCCATGTGGCTGAGAGC	20	20	50.0	55.0	57.8	57.8	0.0	0.0	0.413540	100893	101138	395-433	1.000000	82.7
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	57.7	57.8	7.8	0.0	0.462074	100893	101138	370-408	1.000000	82.7
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	GCTTGTGACTGGAGTGAAGT	CGCTGATGAGCACATTGAAC	20	20	50.0	50.0	57.8	57.8	0.0	4.7	0.416382	100893	101138	414-452	1.000000	82.4
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	20	20	55.0	50.0	57.7	57.8	7.8	4.7	0.464916	100893	101138	389-427	1.000000	82.4
trnI-GAU_p1	trnI-GAU	1	GGGGTTTCTCTCGCTTTTGA	GCCCGTCTTTGTCTCAGTAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.110121	101139	102107	1242-1336	1.000000	86.6
trnI-GAU_p2	trnI-GAU	2	GGGGTTTCTCTCGCTTTTGA	CTCTACGTTCTTGACAGCCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.110121	101139	102107	1258-1352	1.000000	86.5
trnI-GAU_p3	trnI-GAU	3	AGAGTGGAACCCTGAACAGA	GCCCGTCTTTGTCTCAGTAG	20	20	50.0	55.0	57.9	58.0	3.2	0.0	0.105484	101139	102107	1869-1921	0.894737	81.8
trnI-GAU_p4	trnI-GAU	4	AGAGTGGAACCCTGAACAGA	CCTCTACGTTCTTGACAGCC	20	20	50.0	55.0	57.9	58.0	3.2	0.0	0.105484	101139	102107	1886-1938	0.894737	81.7
trnI-GAU_p5	trnI-GAU	5	AGAGTGGAACCCTGAACAGA	CTCTACGTTCTTGACAGCCC	20	20	50.0	55.0	57.9	58.0	3.2	0.0	0.105484	101139	102107	1885-1937	0.894737	81.7
ndhF-rpl32_p1	ndhF-rpl32	1	GCCCACATACGACGAAGATT	CAAGTGAACCGATGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.159781	111895	112649	1075-1171	1.000000	86.9
ndhF-rpl32_p2	ndhF-rpl32	2	GAAGAGGGAATTGTGCCGAT	CAAGTGAACCGATGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.139836	111895	112649	1692-1788	1.000000	86.0
ndhF-rpl32_p3	ndhF-rpl32	3	GAAGAGGGAATTGTGCCGAT	TGTGACAAGTGAACCGATGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.166486	111895	112649	1697-1793	1.000000	85.8
ndhF-rpl32_p4	ndhF-rpl32	4	TCGCGTGAACCAAAAACCTA	CAAGTGAACCGATGGGGAAA	20	20	45.0	50.0	58.0	58.1	5.8	0.0	0.144885	111895	112649	1522-1553	0.789474	73.5
ndhF-rpl32_p5	ndhF-rpl32	5	TAGGTCGCGTGAACCAAAAA	CAAGTGAACCGATGGGGAAA	20	20	45.0	50.0	58.0	58.1	16.8	0.0	0.144885	111895	112649	1526-1557	0.789474	73.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.172709	112824	113491	758-800	1.000000	87.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCGTTGAAGGCTTTTTCGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.174080	112824	113491	759-801	1.000000	87.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGTTGAAGGCTTTTTCGTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.289156	112824	113491	768-810	1.000000	86.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGTTGAAGGCTTTTTCGTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	58.2	57.8	0.0	0.0	0.361174	112824	113491	767-809	1.000000	86.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCGTTGAAGGCTTTTTCGTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.2	57.8	0.0	0.0	0.361174	112824	113491	766-808	1.000000	86.1
ndhD-psaC_p1	ndhD-psaC	1	TTTGACCGCTGTACATTGCT	AAACAACTCGAAGCATGGGT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.127867	116413	116562	558-558	1.000000	82.0
ndhD-psaC_p2	ndhD-psaC	2	TTTGACCGCTGTACATTGCT	GGCATGAAACAACTCGAAGC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.140021	116413	116562	564-564	1.000000	81.9
ndhD-psaC_p3	ndhD-psaC	3	CCTCCCGCAGTGTACAAAAT	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.197527	116413	116562	703-703	1.000000	81.0
ndhD-psaC_p4	ndhD-psaC	4	CTATAATGTGCCTCCCCGTG	AAACAACTCGAAGCATGGGT	20	20	55.0	45.0	57.8	58.0	0.0	0.0	0.188576	116413	116562	913-913	1.000000	80.8
ndhD-psaC_p5	ndhD-psaC	5	GTGGGTGTCTGGTAACCATG	AAACAACTCGAAGCATGGGT	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.197527	116413	116562	896-896	1.000000	80.7
ycf1_p1	ycf1	1	TGTGGTTGTTCTATCAGCCAG	CCTTTTGGTTCTCCCCGAAA	21	20	47.6	50.0	57.9	58.0	2.8	0.0	1.068263	125579	125698	393-393	0.789474	68.7
ycf1_p2	ycf1	2	TGTGGTTGTTCTATCAGCCAG	CCTTAAACCTTGGCACAGGT	21	20	47.6	50.0	57.9	58.0	2.8	13.4	1.062593	125579	125698	514-514	0.789474	68.2
ycf1_p3	ycf1	3	TGTGGTTGTTCTATCAGCCAG	CTCCCCGAAAAAGACGTTCA	21	20	47.6	50.0	57.9	58.1	2.8	0.0	1.184271	125579	125698	383-383	0.789474	68.0
ycf1_p4	ycf1	4	TGTGGTTGTTCTATCAGCCAG	GTTCTCCCCGAAAAAGACGT	21	20	47.6	50.0	57.9	58.1	2.8	0.0	1.182960	125579	125698	386-386	0.789474	67.9
ycf1_p5	ycf1	5	TGTGGTTGTTCTATCAGCCAG	ATTTCCCGAATGGTCCGAAG	21	20	47.6	50.0	57.9	58.0	2.8	0.0	1.096356	125579	125698	661-661	0.789474	67.0
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	GGCACAACGACGCAATTATC	GCTTGTGACTGGAGTGAAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.436522	134970	135213	363-401	1.000000	83.1
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	CTATCCATGTGGCTGAGAGC	GCTTGTGACTGGAGTGAAGT	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.413540	134970	135213	395-433	1.000000	82.7
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	20	20	55.0	55.0	57.8	57.7	0.0	7.8	0.462074	134970	135213	370-408	1.000000	82.7
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	CGCTGATGAGCACATTGAAC	GCTTGTGACTGGAGTGAAGT	20	20	50.0	50.0	57.8	57.8	4.7	0.0	0.416382	134970	135213	414-452	1.000000	82.4
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	57.8	57.7	4.7	7.8	0.464916	134970	135213	389-427	1.000000	82.4