Hotspot

rps15-ycf1

SSC · 125885-126273 · 389 bp

Back to Lagenaria

Pi 0.012
MarkerSeek score 37.80
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
389
variable_sites
7
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCTTGCGAAATAACTGAGCTGA AGCAGTCGTTTTAGTATGCT 40.9 40.0 58.1 54.8 483-484 bp 1.0 41.3
rps15-ycf1_p2 TCTTGCGAAATAACTGAGCTGA GCAGTCGTTTTAGTATGCTAAGG 40.9 43.5 58.1 57.6 482-483 bp 1.0 41.3
rps15-ycf1_p3 CTTGCGAAATAACTGAGCTGA AGCAGTCGTTTTAGTATGCT 42.9 40.0 56.6 54.8 482-483 bp 1.0 41.3
rps15-ycf1_p4 TCTTGCGAAATAACTGAGCTG AGCAGTCGTTTTAGTATGCT 42.9 40.0 56.6 54.8 483-484 bp 1.0 41.3
rps15-ycf1_p5 TCTTGCGAAATAACTGAGCTGA TGGCCCAATTATCGATTAGAAGA 40.9 39.1 58.1 57.4 546-547 bp 1.0 41.2