Hotspot

psbE-petL

LSC · 66620-67884 · 1265 bp

Back to Lagenaria

Pi 0.00264
MarkerSeek score 60.40
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1265
variable_sites
5
pi
0.00264
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGTTCAGACCGA 50.0 47.6 57.8 57.5 1380-1383 bp 1.0 67.2
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AGTCGTATCTTGTTCAGACCG 50.0 47.6 57.8 57.5 1381-1384 bp 1.0 67.1
psbE-petL_p3 GTTCTCCTGTGCTTCCAGAC GTCGTATCTTGTTCAGACCGA 55.0 47.6 58.2 57.5 1375-1378 bp 1.0 66.8
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AGTCGTATCTTGTTCAGACCG 55.0 47.6 58.2 57.5 1376-1379 bp 1.0 66.8
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT GTCGTATCTTGTTCAGACCGA 50.0 47.6 58.4 57.5 1373-1376 bp 1.0 65.6