Hotspot

rpoB-trnC-GCA

LSC · 27936-29115 · 1180 bp

Back to Lagenaria

Pi 0.00228
MarkerSeek score 53.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1180
variable_sites
4
pi
0.00228
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CTCTCATTTCCACCCCCAAG TGTTTGTTGATGAGGCGACA 55.0 45.0 57.9 58.0 1280-1287 bp 1.0 79.5
rpoB-trnC-GCA_p2 CTCTCATTTCCACCCCCAAG CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 1259-1266 bp 1.0 79.2
rpoB-trnC-GCA_p3 CTCTCATTTCCACCCCCAAG TGGGGAAAAAGGGATTTGCA 55.0 45.0 57.9 57.9 1247-1254 bp 1.0 79.0
rpoB-trnC-GCA_p4 TCTCTCATTTCCACCCCCAA TGTTTGTTGATGAGGCGACA 50.0 45.0 58.2 58.0 1281-1288 bp 1.0 78.6
rpoB-trnC-GCA_p5 TTCTCTCATTTCCACCCCCA TGTTTGTTGATGAGGCGACA 50.0 45.0 58.2 58.0 1282-1289 bp 1.0 78.6