Hotspot

ycf1

SSC · 130031-135550 · 5520 bp

Back to Lagarosiphon

Pi 0.132
MarkerSeek score 75.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5520
variable_sites
960
pi
0.132
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.91
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCGCTTGACTTGATTGGTGT GATACCTGGTCCAATACGCA 45.0 50.0 57.7 57.1 451 bp 0.333333 44.1
ycf1_p2 TCGCTTGACTTGATTGGTGT TGATACCTGGTCCAATACGC 45.0 50.0 57.7 57.1 452 bp 0.333333 44.1
ycf1_p3 TCGCTTGACTTGATTGGTGT AGCGAATTCATTGATGGGTCA 45.0 42.9 57.7 58.0 761 bp 0.333333 43.3
ycf1_p4 TCGCTTGACTTGATTGGTGT GGAGCGAATTCATTGATGGG 45.0 50.0 57.7 56.9 763 bp 0.333333 42.7
ycf1_p5 TCGCTTGACTTGATTGGTGT TTGATACCTGGTCCAATACGC 45.0 47.6 57.7 57.8 453 bp 0.333333 41.8