Hotspot

ycf3-trnS-GGA

LSC · 47393-48050 · 658 bp

Back to Lagarosiphon

Pi 0.0569

MarkerSeek score 83.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 54
pi: 0.0569
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGAGATCTAGGCA	CTACACCTTCAACCACTCGG	45.5	55.0	57.2	57.9	700-711 bp	1.0	65.4
ycf3-trnS-GGA_p2	CCATTTATCCGAGATCTAGGCA	GCAGTTCCAATGCTACACCT	45.5	50.0	57.2	58.2	712-723 bp	1.0	64.5
ycf3-trnS-GGA_p3	TCCATTTATCCGAGATCTAGGC	CTACACCTTCAACCACTCGG	45.5	55.0	56.9	57.9	701-712 bp	1.0	63.3
ycf3-trnS-GGA_p4	TCCATTTATCCGAGATCTAGGC	GCAGTTCCAATGCTACACCT	45.5	50.0	56.9	58.2	713-724 bp	1.0	62.4
ycf3-trnS-GGA_p5	CCATTTATCCGAGATCTAGGCA	TGCTACACCTTCAACCACTC	45.5	50.0	57.2	57.5	702-713 bp	1.0	61.7