Hotspot

psbZ-trnG-GCC

LSC · 38844-39419 · 576 bp

Back to Lagarosiphon

Pi 0.0485
MarkerSeek score 80.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
576
variable_sites
37
pi
0.0485
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGTTTTTCTGGTAGCTATCCT CGTCTTCTCCTTGGCAAAGA 38.1 50.0 53.9 57.8 655-672 bp 1.0 48.5
psbZ-trnG-GCC_p2 TGGATTAGTTTTTCTGGTAGCT CGTCTTCTCCTTGGCAAAGA 36.4 50.0 55.1 57.8 661-678 bp 1.0 48.4
psbZ-trnG-GCC_p3 TGGATTGGATTAGTTTTTCTGGT CGTCTTCTCCTTGGCAAAGA 34.8 50.0 56.1 57.8 666-683 bp 1.0 48.4
psbZ-trnG-GCC_p4 TTGGATTAGTTTTTCTGGTAGCT CGTCTTCTCCTTGGCAAAGA 34.8 50.0 55.8 57.8 662-679 bp 1.0 48.4
psbZ-trnG-GCC_p5 GGATTGGATTAGTTTTTCTGGTAGC CGTCTTCTCCTTGGCAAAGA 40.0 50.0 58.2 57.8 665-682 bp 1.0 48.4