Hotspot

trnT-GGU-psbD

LSC · 34535-35646 · 1112 bp

Back to Lagarosiphon

Pi 0.042

MarkerSeek score 80.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 67
pi: 0.042
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1225-1254 bp	1.0	86.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1228-1257 bp	1.0	86.3
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	55.0	50.0	58.1	58.2	1234-1263 bp	1.0	85.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	55.0	50.0	58.1	58.2	1235-1264 bp	1.0	85.1
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	55.0	50.0	58.1	58.2	1236-1265 bp	1.0	85.1