Hotspot

psbM-trnD-GUC

LSC · 31801-32983 · 1183 bp

Back to Lagarosiphon

Pi 0.0467
MarkerSeek score 80.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1183
variable_sites
80
pi
0.0467
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGAACTAGAATAAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 37.5 50.0 57.4 58.5 1301-1341 bp 1.0 47.3
psbM-trnD-GUC_p2 GGAACTAGAATAAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 39.1 50.0 56.0 58.5 1300-1340 bp 1.0 47.3
psbM-trnD-GUC_p3 AGGAACTAGAATAAAGAGTGCAGT GTTCAATTGGTCAGAGCACC 37.5 50.0 57.4 57.0 1302-1342 bp 1.0 47.3
psbM-trnD-GUC_p4 ACTAGAATAAAGAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 39.1 50.0 55.9 58.5 1297-1337 bp 1.0 47.3
psbM-trnD-GUC_p5 AGGAACTAGAATAAAGAGTGCAG TTCAATTGGTCAGAGCACCG 39.1 50.0 55.8 58.5 1301-1341 bp 1.0 47.3