Hotspot

trnS-GCU-trnG-UCC

LSC · 9334-10244 · 911 bp

Back to Lagarosiphon

Pi 0.058

MarkerSeek score 84.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 74
pi: 0.058
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAACAAAACAGAAAGACCCCT	50.0	40.9	58.0	58.0	1025-1424 bp	1.0	71.1
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAACAAAACAGAAAGACCCCT	50.0	40.9	58.0	58.0	1024-1423 bp	1.0	71.1
trnS-GCU-trnG-UCC_p3	AGCAATCCGACGCTTTAGTC	TGAACAAAACAGAAAGACCCCT	50.0	40.9	58.4	58.0	1027-1426 bp	1.0	68.3
trnS-GCU-trnG-UCC_p4	CGACGCTTTAGTCCACTCAG	TGAACAAAACAGAAAGACCCCT	55.0	40.9	58.4	58.0	1020-1419 bp	1.0	68.1
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	TGAACAAAACAGAAAGACCCCT	50.0	40.9	57.6	58.0	1016-1415 bp	1.0	67.9