Hotspot

rps16-trnQ-UUG

LSC · 6365-7783 · 1419 bp

Back to Lagarosiphon

Pi 0.0661
MarkerSeek score 81.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1419
variable_sites
122
pi
0.0661
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.88
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1386-1492 bp 1.0 89.1
rps16-trnQ-UUG_p2 TGATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1397-1503 bp 1.0 88.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1390-1496 bp 1.0 84.4
rps16-trnQ-UUG_p4 ATGATGTCCTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.2 58.1 1398-1504 bp 1.0 84.0
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1378-1484 bp 1.0 84.0