Hotspot

rps3

LSC · 87777-88433 · 657 bp

Back to Lafoensia

Pi 0.00457

MarkerSeek score 61.40

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 3
pi: 0.00457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	ACCCTATAGAATTGAGAATTGCTC	TCATGATATGTATAATAGTGGGGGA	37.5	36.0	56.3	56.4	804 bp	1.0	40.3
rps3_p2	ACCCTATAGAATTGAGAATTGCTC	GCTGAATGGAAAAATAAATACAACGA	37.5	30.8	56.3	57.3	839 bp	1.0	40.3
rps3_p3	ACCCTATAGAATTGAGAATTGCTC	AGCTGAATGGAAAAATAAATACAACG	37.5	30.8	56.3	57.3	840 bp	1.0	40.3
rps3_p4	ACCCTATAGAATTGAGAATTGCT	TCATGATATGTATAATAGTGGGGGA	34.8	36.0	55.2	56.4	804 bp	1.0	40.3
rps3_p5	ACCCTATAGAATTGAGAATTGCT	GCTGAATGGAAAAATAAATACAACGA	34.8	30.8	55.2	57.3	839 bp	1.0	40.3