Hotspot

ndhF

SSC · 114778-117018 · 2241 bp

Back to Lafoensia

Pi 0.0063

MarkerSeek score 47.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 14
pi: 0.0063
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2613-2632 bp	1.0	80.1
ndhF_p2	GCAGAATACCGTCACCCATT	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2723-2742 bp	1.0	79.9
ndhF_p3	ACAGGAACAAGAGGGATCCA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2599-2618 bp	1.0	79.9
ndhF_p4	AAGAGGGATCCACCGAAGAA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2591-2610 bp	1.0	79.9
ndhF_p5	CCGAGTAAATGGAAAGGGGG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	57.9	57.9	2503-2522 bp	1.0	79.9