Hotspot

ndhG-ndhI

SSC · 123961-124362 · 402 bp

Back to Lafoensia

Pi 0.0174

MarkerSeek score 62.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 402
variable_sites: 7
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AACACCACTCCTAGACCTCC	TCAAATTGCTTTGGGTCGTT	55.0	40.0	58.1	56.4	570 bp	1.0	68.0
ndhG-ndhI_p2	AACACCACTCCTAGACCTCC	TCGACTTATGATCGTCACGA	55.0	45.0	58.1	56.2	602 bp	1.0	66.3
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAT	TCAAATTGCTTTGGGTCGTT	45.0	40.0	57.7	56.4	607 bp	1.0	66.2
ndhG-ndhI_p4	AACACCACTCCTAGACCTCC	ATCAAATTGCTTTGGGTCGT	55.0	40.0	58.1	56.2	571 bp	1.0	66.2
ndhG-ndhI_p5	AACACCACTCCTAGACCTCC	TCAAATTGCTTTGGGTCGTTT	55.0	38.1	58.1	57.2	570 bp	1.0	66.0