Hotspot

trnL-UAG-ccsA

SSC · 119286-119385 · 100 bp

Back to Lafoensia

Pi 0.06

MarkerSeek score 79.90

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 100
variable_sites: 6
pi: 0.06
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	GTCCTATGATTTCGTCGACTGA	50.0	45.5	58.0	57.7	280-282 bp	1.0	63.3
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	GTCCTATGATTTCGTCGACTGA	50.0	45.5	58.0	57.7	279-281 bp	1.0	63.3
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	AGTCCTATGATTTCGTCGACTG	50.0	45.5	58.0	57.7	281-283 bp	1.0	63.3
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	AGTCCTATGATTTCGTCGACTG	50.0	45.5	58.0	57.7	280-282 bp	1.0	63.3
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	TGATTTCGTCGACTGATAAGCT	50.0	40.9	58.0	57.6	274-276 bp	1.0	62.7