Hotspot

psbT-pbf1

LSC · 79176-79240 · 65 bp

Back to Lafoensia

Pi 0.138

MarkerSeek score 80.10

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 9
pi: 0.138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	131 bp	1.0	70.9
psbT-pbf1_p2	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	47.6	50.0	57.6	57.4	132 bp	1.0	69.6
psbT-pbf1_p3	CGAGAACCACCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	47.6	50.0	57.6	57.4	154 bp	1.0	69.0
psbT-pbf1_p4	TCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	47.6	55.0	57.3	58.4	132 bp	1.0	68.6
psbT-pbf1_p5	TCGAGAACCACCTAAAGTTCC	GGCAACCCTCTCAACAACTA	47.6	50.0	57.3	57.4	155 bp	1.0	66.8