Hotspot

psbM-trnD-GUC

LSC · 31184-32348 · 1165 bp

Back to Lafoensia

Pi 0.00773

MarkerSeek score 56.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 9
pi: 0.00773
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCGCCTTGACTGACTGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.7	58.5	1330-1331 bp	1.0	74.1
psbM-trnD-GUC_p2	AATCGCCTTGACTGACTGTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.4	58.5	1332-1333 bp	1.0	72.3
psbM-trnD-GUC_p3	ATCGCCTTGACTGACTGTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.4	58.5	1331-1332 bp	1.0	72.3
psbM-trnD-GUC_p4	TCGCCTTGACTGACTGTTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.7	57.0	1331-1332 bp	1.0	70.0
psbM-trnD-GUC_p5	ATCGCCTTGACTGACTGTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.4	57.0	1332-1333 bp	1.0	68.2