Hotspot

trnC-GCA-petN

LSC · 29311-30225 · 915 bp

Back to Lafoensia

Pi 0.00874

MarkerSeek score 56.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 8
pi: 0.00874
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	972 bp	1.0	78.5
trnC-GCA-petN_p2	GGTATCATTTTGGCGGCATG	CAGCCCAAGCAAGACTTACT	50.0	50.0	57.9	57.8	1025 bp	1.0	78.0
trnC-GCA-petN_p3	GTATCATTTTGGCGGCATGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	57.9	57.8	1024 bp	1.0	78.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CTACCATTAACGCAGCCCAA	50.0	50.0	58.1	58.3	984 bp	1.0	78.0
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TACCATTAACGCAGCCCAAG	50.0	50.0	58.1	58.3	983 bp	1.0	78.0