Hotspot

rps2

LSC · 23851-24561 · 711 bp

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Pi 0

MarkerSeek score 26.70

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TGACCTACTTCCACAGCAGA	TTTTTCACCCGGCGAACTAA	50.0	45.0	58.0	58.0	1683-1684 bp	1.0	79.7
rps2_p2	TGACCTACTTCCACAGCAGA	GCCAATCCTCGGTAGACAAG	50.0	55.0	58.0	58.1	1169-1170 bp	1.0	79.6
rps2_p3	TGACCTACTTCCACAGCAGA	TGCCTTGGAAGAAGCGATTT	50.0	45.0	58.0	58.1	1632-1633 bp	1.0	79.3
rps2_p4	CGAAAGTGGGAATGGTTTGC	TTTTTCACCCGGCGAACTAA	50.0	45.0	57.9	58.0	1814-1815 bp	1.0	79.3
rps2_p5	GAAAGTGGGAATGGTTTGCG	TTTTTCACCCGGCGAACTAA	50.0	45.0	57.9	58.0	1813-1814 bp	1.0	79.3