Hotspot

rpoB

LSC · 31981-35193 · 3213 bp

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Pi 0.000934

MarkerSeek score 55.80

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 3
pi: 0.000934
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GCACCGTCCGCTAAAATTTG	GTTATAGCCGAACACGAGGG	50.0	55.0	58.4	58.2	191 bp	1.0	76.1
rpoB_p2	GCACCGTCCGCTAAAATTTG	TATAGCCGAACACGAGGGAA	50.0	50.0	58.4	58.2	189 bp	1.0	75.5
rpoB_p3	GCACCGTCCGCTAAAATTTG	TTATAGCCGAACACGAGGGA	50.0	50.0	58.4	58.2	190 bp	1.0	75.5
rpoB_p4	AGCACCGTCCGCTAAAATTT	GTTATAGCCGAACACGAGGG	45.0	55.0	58.5	58.2	192 bp	1.0	75.5
rpoB_p5	GCACCGTCCGCTAAAATTTG	TTCCCGTTATAGCCGAACAC	50.0	50.0	58.4	57.7	196 bp	1.0	74.9