Hotspot

ndhJ

LSC · 12039-12515 · 477 bp

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Pi 0.0021

MarkerSeek score 47.00

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: LSC
length_bp: 477
variable_sites: 1
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	AGACCGGATTCTTTTGTCCA	CTGGGATTGTAGAAATGCGGA	45.0	47.6	56.8	58.1	629-630 bp	1.0	61.5
ndhJ_p2	AGACCGGATTCTTTTGTCCA	TCTGGGATTGTAGAAATGCGG	45.0	47.6	56.8	58.1	630-631 bp	1.0	61.5
ndhJ_p3	AGACCGGATTCTTTTGTCCA	TGGGATTGTAGAAATGCGGA	45.0	45.0	56.8	56.9	628-629 bp	1.0	61.4
ndhJ_p4	AGACCGGATTCTTTTGTCCAA	CTGGGATTGTAGAAATGCGGA	42.9	47.6	57.5	58.1	629-630 bp	1.0	59.5
ndhJ_p5	AGACCGGATTCTTTTGTCCAA	TCTGGGATTGTAGAAATGCGG	42.9	47.6	57.5	58.1	630-631 bp	1.0	59.5