Hotspot

psbE-petL

LSC · 62879-64276 · 1398 bp

Back to Laburnum

Pi 0.00215

MarkerSeek score 60.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1398
variable_sites: 3
pi: 0.00215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1539-1543 bp	1.0	79.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1541-1545 bp	1.0	79.9
psbE-petL_p3	ATAGCCAACCCGCAATGAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	57.9	58.0	1544-1548 bp	1.0	79.8
psbE-petL_p4	CAAATAGCCAACCCGCAATG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.1	58.0	1547-1551 bp	1.0	79.8
psbE-petL_p5	ATAGCCAACCCGCAATGAAT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	57.9	58.0	1546-1550 bp	1.0	79.7