Hotspot

trnS-GCU-trnT-CGU

LSC · 15989-16792 · 804 bp

Back to Laburnum

Pi 0.00125

MarkerSeek score 59.60

Primer available yes

Label trnS-GCU-trnT-CGU

Feature Metadata

label_name: trnS-GCU-trnT-CGU
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 1
pi: 0.00125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnT-CGU_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	917-936 bp	1.0	74.5
trnS-GCU-trnT-CGU_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	916-935 bp	1.0	74.4
trnS-GCU-trnT-CGU_p3	GTCAGTAACGCGGAAAGAGA	TGAATCAAACGAGGGATCCC	50.0	50.0	57.7	57.3	979-998 bp	1.0	71.7
trnS-GCU-trnT-CGU_p4	AGCAATCCGACGCTTTAGTC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.4	57.3	919-938 bp	1.0	71.6
trnS-GCU-trnT-CGU_p5	CAGTAACGCGGAAAGAGAGG	TGAATCAAACGAGGGATCCC	55.0	50.0	58.4	57.3	977-996 bp	1.0	71.5